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The older a sample is, the less (the period of time after which half of a given sample will have decayed) is about 5,730 years, the oldest dates that can be reliably measured by this process date to around 50,000 years ago, although special preparation methods occasionally permit accurate analysis of older samples.

Radiocarbon dating has allowed key transitions in prehistory to be dated, such as the end of the last ice age, and the beginning of the Neolithic and Bronze Age in different regions.

Animals eat the plants, and ultimately the radiocarbon is distributed throughout the biosphere.

The ratio of λ is a constant that depends on the particular isotope; for a given isotope it is equal to the reciprocal of the mean-life – i.e.

the average or expected time a given atom will survive before undergoing radioactive decay. The calculations involve several steps and include an intermediate value called the "radiocarbon age", which is the age in "radiocarbon years" of the sample: an age quoted in radiocarbon years means that no calibration curve has been used − the calculations for radiocarbon years assume that the , which for more than a decade after Libby's initial work was thought to be 5,568 years.

This was revised in the early 1960s to 5,730 years, which meant that many calculated dates in papers published prior to this were incorrect (the error in the half-life is about 3%).

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