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Ch AT-Ch R2-EYFP line 6 mice also consume more food and water during the dark cycle compared to control mice. There are approximately 50 copies of the transgene present.

Compared to wildtype animals, Ch AT-Ch R2-EYFP line 6 mice show no changes in metabolic profile, locomotor activity in a novel environment, anxiety-like behavior, depression-like behavior, gross sensorimotor function, and ability to use cues to learn a task. Of note, the donating investigator may not have fixed the Y chromosome to the C57BL/6J genetic background during backcrossing.

As such, illuminating mh Ch R2-expressing neurons with blue light (450-490 nm) leads to rapid and reversible photostimulation of action potential firing/neural activity in these cells.

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VACh T protein is overexpressed in the hippocampus and brainstem.

EYFP expression is visible by direct fluorescence (epifluorescence microscope).




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